Bioenergetics Spreadsheet

Download our Bioenergetics Spreadsheet and Instructional PDF to easily convert your seahorse data into bioenergetics figures of merit. This spreadsheet will simplify your analysis and allow you to visually interrogate single or comparative datasets. Our instructional PDF will:

  • walk you through entering your data into the spreadsheet
  • explain the graphs you will see
  • provide instructions for setting up your seahorse experiment to generate data in a way that is easily incorporated into the spreadsheet

The spreadsheet calculations are based off of Mookerjee and Brand’s paper Quantifying Intracellular Rates of Glycolytic and Oxidative ATP Production and Consumption Using Extracellular Flux Measurements and have been updated to reflect his 2019 corrections.

Bioenergetics Spreadsheet

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